Fig. 7.

H. diminuta extract (HdE) suppression of neutrophil chemotaxis occurs via p38 MAP kinase. a Murine bone marrow neutrophil (2 × 10⁵, NØs) migration in a transwell (8 μm pore size filter) in response to the peptide WKYMVm (1 μM) is completely blocked by pre-treatment (30 min, 37°C) with the selective pharmacologic inhibitor of the p38 mitogen-activated protein kinase (MAPK) pathway, SB20350 (2.5 μM) (data is mean ± SD; each datum point is the average of 2 NØ replicates from an individual mouse; * and ^# p < 0.05 compared to control (con) or WKYMVm only, respectively, by one-way analysis of variance with Tukey's post-test). b Immunoblots show that activation of p38 in NØs and the downstream signal heat-shock protein (HSP) 27 by WKYMVm is inhibited by the co-treatment with HdE (100 μg/mL; 10 min, 37°C; representative immunoblot and densitometric analysis where data are mean ± SD, ^# p < 0.05 compared to ­WKYMVm only, by Kruskal-Wallis test with Dunn's post-test).