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. 2019 Aug 27;11(16):6371–6384. doi: 10.18632/aging.102194

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Copyright © 2019 Cai et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY 3.0) License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Contribution of miR-27a expression to the growth of IL-1β-triggered chondrocytes. (A) Expression levels of miR-27a in IL-1β-treated chondrocytes that underwent transfection with the miR-27a inhibitor and miR-Scr were evaluated by Q-PCR. (B) The proliferation rate of IL-1β-treated chondrocytes was measured at 24, 48, and 72 h following their transfection using the MTT test. (C) Soft agar colony generation assay of IL-1β-treated chondrocytes transfected with the miR-27a inhibitor and miR-Scr. The lower panel indicates the number of colonies in each group. The results are described as the mean ± SD. *P < 0.05, **P < 0.01 vs. indicated group.