Figure 1.

MLN4924 effectively induces cell death in Ph+ leukemia cells. In vitro MLN4924-sensitivity of B-ALL cell lines (a) and MM cell lines (b). Cells were treated for 3 days with the indicated concentrations of MLN4924, cell death was determined by 7-AAD staining and flow-cytometry, and specific cell death was calculated as indicated in the materials and methods section. Three independent experiments were performed and cultures were done in quadruplicate, means ± SEM are shown. EC₅₀ values were calculated by non-linear regression.