Figure 2.

MLN4924 synergizes with imatinib and HU for the induction of cell death in Ph+ leukemia cells. (a) Ph+ leukemia cell lines were treated for 3 days with various concentrations of MLN4924 and imatinib at a fixed ratio of 1:100. Three independent experiments were performed, cultures were done in quadruplicate, means ± SEM are shown. (b) SUP15 and BV173 cells were treated for 3 days with indicated concentrations of MLN4924 and ponatinib at a fixed ratio of 5:1. Three independent experiments were performed, cultures were done in triplicate, means ± SEM of a representative experiment are shown. (c) Ph+ leukemia cell lines were treated overnight with various concentrations of MLN4924 and HU at a fixed ratio of 1:1000, as indicated. Three independent experiments were performed, cultures were performed in quadruplicate, means ± SEM are shown. Combination index (CI) values are given for each relevant data point.