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. 2019 Jul 26;18(18):2293–2306. doi: 10.1080/15384101.2019.1644913

Figure 3.

Figure 3.

Regulation of cell cycle and cell proliferation by INK4 family genes via CDK4 and cyclin D1.

(A) Flow cytometry analysis examining cell cycle changes after altering INK4 family gene expression. (B) Flow cytometry analysis examining cell cycle changes after altering CDK4 or cyclin D1 expression. CDK4-oe: CDK4 overexpression and cyclin D1-oe: cyclin D1 overexpression. (C) Flow cytometry analysis examining cell cycle changes after altering INK4 family gene expression, and CDK4 and cyclin D1 expression. (D) MTT reduction assay evaluating proliferation at 0 h, 24 h, 36 h, and 48 h after altering INK4 family gene expression. (E) Flow cytometry analysis examining cell proliferation at 0 h, 24 h, 36 h, and 48 h after altering INK4 family expression. (F) MTT reduction assay evaluating proliferation at 0 h, 24 h, 36 h, and 48 h after altering CDK4 and cyclin D1 expression. (G) Flow cytometry analysis examining cell proliferation at 0 h, 24 h, 36 h, and 48 h after altering CDK4 and cyclin D1 expression. (H) Transwell cell migration assay examining the effects of INK4 family genes and CDK4 and cyclin D1 on tumor cell migration.