Figure 8.

Upregulated miR-140-5p inhibits apoptosis of synoviocytes of rats with KOA. a, cell apoptosis was detected by Hoechst 33,342 staining; b, cell apoptosis was detected by flow cytometry and comparison of apoptosis rate; c, the expression of Bcl-2 and Bax was detected by RT-qPCR; d, protein bands of Bcl-2 and Bax; E, the statistical results of protein expression of Bcl-2 and Bax in each group by Western blot analysis; * P < 0.05 vs the normal group, # P < 0.05 vs the ACLT group. The experiment was repeated for three times. The measurement data conforming to the normal distribution were expressed as mean ± standard deviation. ANOVA was used for comparisons among multiple groups. Tukey’s multiple comparisons test was used for pairwise comparisons after ANOVA.