Table 1.
Gene | Primer sequence (5’-3’) |
---|---|
miR-140-5p U6 TLR4 Myd88 NF-κB Bcl-2 Bax IL-1β TNF-α |
F: 5’- ACACTCCAGCTGGGAGGCGGGGCGCCGCGGGA- 3’ R: 5’- CTCAACTGGTGTCGTGGA- 3’ F: 5’- CTCGCTTCGGCAGCACA- 3’ R: 5’- AACGCTTCACGAATTTGCGT- 3’ F: 5’- ACAAACGCCGGAACTTTTCG- 3’ R: 5’- GTCGGACACACACAACTTAAGC- 3’ F: 5’- TTGCCAGCGAGCTAATTGAG- 3’ R: 5’- ACAGGCTGAGTGCAAACTTG- 3’ F: 5’-TGTCTGCACCTGTTCCAAAG- 3’ R: 5’-TCAGCATCAAACTGCAGGTG- 3’ F: 5’-ACTTCTCTCGTCGCTACCGTCG- 3’ R: 5’- CCCTGAAGAGTTCCTCCACCACC- 3’ F: 5’- TGGGCTGGACACTGGACTTC- 3’ R: 5’- CTTCCAGATGGTGAGTGAGGC- 3’ F: 5’- GACTTCACCATGGAACCCGT- 3’ R: 5’- GGAGACTGCCCATTCTCGAC- 3’ F: 5’-TTACAGGAAGTCCCTCACCCTC- 3’ R: 5’- CCCAGAGCCACAATTCCCTT- 3’ |
β-actin | F: 5’-TTACAGGAAGTCCCTCACCCTC- 3’ |
R: 5’-CCCAGAGCCACAATTCCCTT-3’ |
Note: F, forward; R, reverse; miR-140-5p, microRNA-140-5p; TLR4, toll-like receptor 4; NF-κB, nuclear factor kappa-B; IL-1β, interleukin (IL)-1β; TNF-α, tumor necrosis factor-α.