CASC15 upregulation in lung cancer cells promotes cell proliferation and invasion.
(a). qRT-PCR analysis of CASC15 levels in lung cancer cells, including A549, H1299, H1975 and SPC-A-1. Normal lung cell, BEAS-2B was used as control. (b). Evaluation of CASC15 silencing using three siRNA specific to CASC15, namely si-CASC15-1, siCASC15-2, si-CASC15-3. Non-transfected cell (control) and cells transfected with si-NC were used as controls. (c). CCK-8 assay of cell proliferation. (d). Colony formation assay. (e). Scratch wound assay of cell migration. (f). Transwell assay of cell invasion.