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. Author manuscript; available in PMC: 2019 Sep 11.
Published in final edited form as: Free Radic Biol Med. 2019 Mar 1;135:132–143. doi: 10.1016/j.freeradbiomed.2019.02.029

Fig. 6. Effect of cinnamaldehyde (CNM) on mRNA and protein expression of nNOS α.

Fig. 6.

(A-D), nNOSα dimerization (E, F) and GCH-1 (G-J) in WT and Nrf2 KO female mice gastric neuromuscular tissues. Representative immunoblot and densitometric analysis data for (A) nNOSα mRNA expression in WT; (B) nNOSα mRNA expression in Nrf2 KO, (C) nNOSα protein expression in WT; (D) nNOSα protein expression in Nrf2 KO, (E) nNOSα dimerization in WT and (F) nNOSα dimerization in Nrf2 KO; (G) GCH-1 mRNA expression in WT; (H) GCH-1 mRNA expression in Nrf2 KO; (I) GCH-1 protein expression in WT and (J) GCH-1 protein expression in Nrf2 KO in female mice gastric neuromuscular tissue. Stripped blots were re-probed with β-actin. Data were normalized with housekeeping gene or protein (β-actin). Bar graphs showed a ratio of target gene or protein with β-actin. Data were analyzed using one way ANOVA by using graph pad prism software. The values are mean ± SEM (n = 4). *p < 0.05 compared with control group; #p < compared with the diabetic group.