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. 2019 Sep 11;15(9):e1007289. doi: 10.1371/journal.pcbi.1007289

Fig 4. Ex vivo live imaging of chromocenter (CC) clusters in mouse rod cells 15 days after birth (P15).

Fig 4

(A) Live imaging system (see the Supplemental Experimental Procedures for details). ONL: outer nuclear layer. (B) Time-lapse single plane images of a single rod-cell nucleus in a P15 mouse. The white dashed lines represent the nuclear edges. Scale bars: 2 μm. (C) Schematic cell nucleus with the ROI (white frame, left panel) used to construct the kymograph (right panel). (D) Correlated change in the nucleus width and distance between CC clusters. Data are derived from the kymograph in panel (C). (E) A representative projected-fluorescence image of the rod-cell nuclei from a P15 mouse (S7 Movie). Center of mass in CC clusters of cells are represented by colored balls. Color-coded lines represent trajectories of CC clusters. Scale bar: 5 μm. (F, G) Dynamics of CC distances within individual nuclei (gray lines) and averaged CC distances (blue lines). Error bars represent SEM. N = 142 from 20 cells.