Fig 1. Increased expression of factors in the CWI signaling pathway counteracts the Ts phenotype of W303-derived elp3Δ cells.

(A) Growth of the elp3Δ (UMY3269) strain carrying the indicated high-copy (h.c.) or low-copy (l.c.) LEU2 plasmids. The high copy plasmid carrying the tK(UUU) and tQ(UUG) genes [74] is abbreviated as h.c. tK+tQ. Cells were grown over-night at 30°C in liquid synthetic complete medium lacking leucine (SC-leu), serially diluted, spotted on SC-leu plates, and incubated at 30°C or 37°C for 3 days. (B) Northern analysis of total RNA isolated from elp3Δ (UMY3269) cells carrying the indicated plasmids. The cells were grown in SC-leu medium at 30°C or 37°C. The blot was probed for ${\text{tRNA}}_{\text{UUU}}^{\text{Lys}}$, ${\text{tRNA}}_{\text{UUG}}^{\text{Gln}}$, ${\text{tRNA}}_{\text{i}}^{\text{Met}}$, and 5.8S rRNA using radiolabeled oligonucleotides. 5.8S rRNA serves as the loading control.