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. 2019 Aug 29;15(8):e1008117. doi: 10.1371/journal.pgen.1008117

Fig 2. The growth phenotypes elp3Δ cells are modulated by the allele at the SSD1 locus.

Fig 2

(A) Growth of elp3Δ mutants in the W303 and S288C genetic backgrounds. The wild-type (W303-1A and BY4741) and elp3Δ strains (UMY3269 and MJY1036) were streaked on SC plates and incubated at 30°C or 37°C for 3 days. (B) Effects of the ssd1-d2/SSD1 alleles on the growth of elp3Δ strains in the W303 and S288C genetic backgrounds. The ssd1-d2 (W303-1A and UMY4432), SSD1 (UMY3385 and BY4741), ssd1-d2 elp3Δ (UMY3269 and UMY4439) and SSD1 elp3Δ (UMY4456 and MJY1036) strains were grown over-night at 30°C in liquid SC medium, serially diluted, spotted on SC plates, and incubated at 30°C or 37°C for 3 days. (C) Effects of increased PKC1 dosage on the growth of elp3Δ ssd1-d2 and elp3Δ SSD1 strains. The relevant strains (UMY3269, UMY4456, UMY4439, and MJY1036) carrying the indicated plasmids were grown over-night at 30°C in liquid SC-leu medium, serially diluted, spotted on SC-leu plates, and incubated for 3 days at 30°C or 37°C. (D) Influence of the ssd1-d2 allele on growth phenotypes induced by various stress-inducing agents. The strains from B were grown over-night at 30°C in liquid SC medium, serially diluted, and spotted on SC plates and SC plates supplemented with caffeine, rapamycin, hydroxyurea or diamide. The plates were incubated for 3 days at 30°C.