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. 2019 Aug 29;15(8):e1008117. doi: 10.1371/journal.pgen.1008117

Fig 4. The growth and protein homeostasis defects of elp3Δ ncs2Δ cells are augmented by the ssd1-d2 allele.

Fig 4

(A) Influence of the ssd1-d2 allele on the growth of elp3Δ ncs2Δ cells. The ssd1-d2 elp3Δ ncs2Δ (UMY4454 and MJY1159) and SSD1 elp3Δ ncs2Δ (UMY4467 and MJY1058) strains carrying the l.c. URA3 plasmid pRS316-ELP3 were grown over-night at 30°C in SC medium, serially diluted, spotted on SC and SC+5-FOA plates, and incubated for 3 days at 30°C. (B) Growth of S288C-derived SSD1 elp3Δ ncs2Δ and ssd1-d2 elp3Δ ncs2Δ strains. The wild-type (BY4741 and UMY4432) and elp3Δ ncs2Δ strains (MJY1058 and UMY4449) were streaked on a SC plate and incubated at 30°C for 2 days. (C) Effects of the ssd1-d2 allele on protein aggregation in elp3Δ ncs2Δ cells. Total protein and protein aggregates was analyzed from the ssd1-d2 (UMY4432), ssd1-d2 elp3Δ ncs2Δ (UMY4449), SSD1 (BY4741) and SSD1 elp3Δ ncs2Δ (MJY1058) strains grown in SC medium at 30°C. The gel is a representative of two independent experiments.