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IFN-γ secretion by splenocytes is dependent on TLR4, MyD88 and TRIF. Splenocytes derived from WT, Tlr4-/-, Myd88-/- and TRIF mutant mice were stimulated with different concentrations of growth-arrested K. pneumoniae and LPS derived from E. coli or K. pneumoniae (n = 4–6 for each condition). IFN-γ levels were determined after 48 h. Data are expressed as mean (SE). ** p < 0.01, Mann-Whitney U test (performed post hoc after Kruskal-Wallis test).
