Fig. 5.

TRIF mutant mice have attenuated liver injury that increases after rIFN-γ treatment. WT and TRIF mutant mice were infected with about 10⁴ CFU K. pneumonia; 50 ng rIFN-γ or vehicle was administered intranasally 30 min before infection and 24 h afterwards. Mice were sacrificed after 48 h of infection. AST (a) and ALT (b) plasma levels and liver histopathology were scored (see Methods) (c) expressed as box-and-whisker diagrams depicting the smallest observation, lower quartile, median, upper quartile and largest observation. * p < 0.05, ** p < 0.01, *** p < 0.001, Mann-Whitney U test (performed post hoc after Kruskal-Wallis test).