Fig. 4.

DNA-induced rapid cell death is not prevented by caspase inhibition. a Actinomycin D-induced apoptosis is prevented by Z-VAD-FMK. S2 cells were treated with or without actinomycin D with either 50 µM of Z-VAD-FMK or DMSO vehicle control for 6 h, and analysed for loss of membrane integrity by PI staining and flow cytometry. b DNA-induced cell death in S2 cells is not affected by Z-VAD-FMK. S2 cells were pretreated with 50 µM of Z-VAD-FMK or DMSO vehicle control for 1 h prior to electroporation with 10 µg of CT DNA. After 30 min they were stained with PI and analysed by flow cytometry. Data are representative of 2 experiments.