Table 1.
Surface markers | WT | KO |
---|---|---|
CD40 | ||
% | 57.2±2.9 | 39.1±3.1* |
MFI | 178.7±2.5 | 115.9±2.0*** |
CD80 | ||
% | 29.6±2.3 | 21.4±1.6* |
MFI | 36.8±2.1 | 27.9±2.3 |
CD86 | ||
% | 14.9±1.0 | 22.5±1.0** |
MFI | 15.3±1.3 | 23.9±2.4* |
MHC-II | ||
% | 91.0±1.1 | 86.9±1.0* |
MFI | 571.8±13.5 | 500.7±8.8* |
Three mice from each group were sacrificed at day 3 after Cpn infection. CD11c+ lung cells were isolated by CD11c-magnetic microbead selection. CD11chi non-autofluorescent lung cells were gated as LDCs as described in figure 1a. The surface molecule expression on LDCs was analyzed by flow cytometry. Statistical analyses were performed using a Student's t test. Data are shown as mean ± SD. One of the 2 independent experiments with similar results is shown. MFI = Mean fluorescence intensity.
p <0.05;
p <0.01;
p <0.001.