Fig. 2.

Tep4 mutants are resistant to P. luminescens during the initial phases of infection. Survival ofloss-of-function tep4 mutants and control flies (yw) after intrathoracic injection with P. luminescens (Pl) (a) or P. asymbiotica (Pa) (b) (20 flies/experimental condition). Injections with PBS served as injury control. Survival was monitored for 48 h at 6-hour intervals and the black dotted line represents 50% survival. CFU of P. luminescens (a) or P. asymbiotica (b) at 6 and 18 h after injection were determined by quantitative PCR of mcf-1 (P. luminescens) and tccC3 (P. asymbiotica) in tep4 mutants and their background control yw flies (5 individuals/experimental condition). * p< 0.05, ** p < 0.01, *** p < 0.001, significant differences. The means from 3 independent experiments are shown and error bars represent standard errors (survival) and standard deviation (bacterial load).