Fig. 4.

Melanization response and PO activity are elevated in tep4 mutants upon Photorhabdus infection. a Melanization of the wound site in tep4 loss-of-function mutant flies and their background control strain (yw) is shown 3 h after injection with PBS, P. luminescens or P. asymbiotica. Arrows indicate the site of injury. ×10. b PO activity in the hemolymph plasma of tep4 mutants and control flies (yw) 3 h after injection with PBS, P. luminescens (Pl) or P. asymbiotica (Pa) (n = 20 flies), as measured by the OD at 492 nm after incubation with L-DOPA. Values represent the means from 3 biological replicates and error bars represent standard deviations. *** p < 0.001, significant differences.