Fig. 7.

Protein content and immunostimulatory properties of concentrated bacterial supernatants enriched in vesicle-like structures. 5448 was incubated in the absence or presence of 1 and 10 μM LL-37 for 10 h and cell-free, concentrated supernatants were prepared. a SEM analyses revealed the presence of vesicle-like structures in LL-37-exposed bacterial supernatants. Scale bar: 200 nm. b Proteins in the concentrated bacterial supernatants were separated on SDS gel and detected by silver staining. The same concentrated supernatants were added in different concentrations to cultures with primary neutrophils. d After 2 h of incubation, the neutrophil supernatants were analysed for resistin and MPO. Lysates of neutrophils served as a positive (+) and the supernatant of neutrophils incubated with cell culture media as negative (-) control (Ctr). The bars show the mean ± SD of 3-4 experiments. conc. SUPs stim. = Concentrated supernatants stimulated.