Fig. 2.
IL-17A enhances recruitment of arginase-1-producing CD11b+Gr1intF4/80+ cells to K14.E7 mouse skin following DNCB application. a Arginase activity in K14.E7 and E7.IL17−/- mouse skin after 24 h of DNCB treatment. b, c Expression of arginase-1 (b) and arginase-2 (c) mRNA transcripts in K14.E7 and E7.IL17− /- mouse skin following DNCB application determined by real-time PCR. d Immunohistochemical analysis of F4/80+ macrophages in K14.E7 or E7.IL17−/- skin exposed to DNCB. Stained sections are representative of 4 mice/group, original magnification ×200, scale bar = 100 μm. e Absolute numbers of infiltrating CD11b+Gr1+F4/80+ cells in K14.E7 and E7.IL17−/- skin 1 day after DNCB treatment was determined by flow cytometry. Data are means ± SEM and are pooled from two independent experiments, each with 4–7 mice/group. * p < 0.05, ** p < 0.001, *** p < 0.0001. n.s. = Non-significant.