Fig. 6.

mpeg1 does not play a role in phagocytosis of M. marinum or in leukocyte migration towards local inflammation. a Quantification of M. marinum phagocytosis. mpeg1 morphants and their controls were injected with an mCherry-expressing M. marinum Mma20 strain (180 cfu), fixed at 5, 10, 20, 30 and 40 min post-infection, and stained with L-plastin Ab to label leukocytes. Intra- and extra-cellular bacteria were counted over the yolk sac and results are presented as percentages of phagocytosed Mma20. b Representative images of untreated and copper sulphate-treated control and mpeg1 morphant 3 dpf embryos. Embryos were immunolabelled with Ab against the general leukocyte marker L-plastin (red signal) in combination with a neutrophil-specific Mpx TSA staining (green signal). White arrows indicate accumulation of leukocytes at the local inflammation sites at the neuromasts. n.s. = Not significant; mo1 = morpholino 1.