Fig. 6.

p38 and ERK mediate increased reactivity of bronchial epithelial cells to LPS and pIC upon ER stress induction. BEAS-2B (a, b) and hpBrEpC cells (c, d) were pretreated with the inhibitors of p38 (SB203580, 10 µM), ERK (U0126, 10 µM), and JNK (SP600125, 10 µM) for 15 min and then with DMSO or thapsigargin (Thaps) for 1 h. Subsequently, cells were stimulated with LPS or pIC for 5 h. Secreted IL6 (a, c) and IL8 (b, d) levels were determined by ELISA (n = 4-6). Data are shown as the mean + SD.