Table 2.

Functional analyses of CTLD genes/proteins in C. elegans

Gene/protein	Approach	Phenotypic measurement	Phenotype/result	Pathogen	Ref.
clec-17	RNAi knockdown	infection	hyperdeformed anal region (Dar) response, severe constipation	MN	53
clec-39	knockout mutant	survival	higher susceptibility
clec-49		egg laying	lower egg laying
CLEC-39	ELISA-based assay	binding assays	binding to live/dead pathogen	SM	55
CLEC-49	with recombinant proteins
clec-52	promoter fusion
reporter	fluorescence	expression in intestine	SA	52
		induction
	promoter fusion
reporter	fluorescence	expression in intestine
		induction
clec-60	RNAi knockdown	infection	hyperdeformed anal region (Dar) response,	MN	53
			severe constipation
	promoter fusion
reporter	fluorescence	expression in intestine	SA	52
		induction
clec-60/61	overexpression	survival	higher susceptibility	PA	52
cluster
clec-65	RNAi knockdown	survival	higher susceptibility	EC	51
clec-67	promoter fusion	fluorescence	expression in intestine	SE	56
	reporter	induction
clec-70	RNAi knockdown	survival	higher susceptibility	SA	52
	promoter fusion	fluorescence	expression in intestine
	reporter	induction
clec-70/71	overexpression	survival	higher resistance	SA	52
cluster			higher susceptibility	PA
CLEC-79	glycoconjugate	binding	binding to Galb1-3GalNAc		54
	microarray	assay
	with recombinant
	protein
clec-85	promoter fusion	fluorescence	dependence on immune components	SA	78
	reporter	intensity	tir-1, nsy-1, dbl-1, daf-16	PA
clec-86	RNAi knockdown	infection	hyperdeformed anal region (Dar) response,	MN	53
			severe constipation
clec-174	RNAi knockdown	survival	higher susceptibility	VC	50

EC = E. coli strain LF82; SE = S. enterica. For other abbreviations, see the legend to figure 1.