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. 2016 Mar 3;8(3):269–283. doi: 10.1159/000443646

Fig. 4.

Fig. 4

Far Western analysis to detect C1q binding to the major Omps of V. cholerae. a SDS-PAGE and immunoblot analysis to detect indirect binding of C1q to OmpU. Lane 1, ΔompT; lane 2, ΔompU; lane 3, ΔompA; lane 4, wild-type A1552; lane 5, ΔtoxR. The arrow shows the C1q signal on the OmpU protein. Molecular weight markers are shown to the left of the membrane. b SDS-PAGE and immunoblot analysis of antibody-dependent binding of C1q on OmpU after IdeS treatment. Lane 1, ΔompT; lane 2, ΔompU; lane 3, ΔompA; lane 4, wild-type A1552; lane 5, ΔtoxR. Lane 6 was loaded with 100 ng of purified C1q protein. The arrow shows the immunoreaction band of C1q protein. Molecular weight markers are shown to the left of the membrane.