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. 2016 Mar 3;8(3):269–283. doi: 10.1159/000443646

Fig. 5.

Fig. 5

Serum sensitivity assay with V. cholerae monitoring the effects of IdeS, OMVs and antisera raised against OmpU, C1q, IgG or Hcp. a Panel 1: blue column, percent survival of V. cholerae strain 569B in NHS; red column, percent survival of V. cholerae strain 569B in NHS treated with IdeS. Panel 2: blue column, percent survival of 569B in NHS and OMVs from wild-type strain A1552. Panel 3: blue column, percent survival of 569B in NHS with OMVs from the ΔompU mutant. Panel 4: blue column, percent survival of 569B in NHS with OMVs from the ΔompT mutant. Panel 5: blue column, percent survival of 569B in NHS with OMVs from ΔompA mutant. Panel 6: blue column, percent survival of 569B in NHS with OMVs from ΔtoxR mutant. Panels 2-6: red columns represent percent survival of the same strains in the presence of NHS+OMVs+IdeS (colors refer to the online version only). Values represent the mean ± SD of three independent experiments. The asterisk indicates the survival percentage of V. cholerae 569B in NHS treated with OMVs versus NHS without treatment with OMVs (p < 0.05). b Blue columns represent the survival of V. cholerae wild-type strain A1552 and its derivatives in NHS alone. Red columns show the percent survival of A1552 and its derivatives treated in NHS and anti-OmpU antiserum. Green columns represent the survival percentage of A1552 and its derivatives in NHS and anti-C1q antiserum. Violet columns show the percent survival of A1552 and its derivatives in NHS and anti-IgG antibody. Gray columns show the percent survival of A1552 and its derivatives in NHS and anti-Hcp antibody (colors refer to the online version only). The strain genotypes are indicated below the columns. Values represent the mean ± SD of three independent experiments. Single asterisks indicate wild-type V. cholerae A1552 in NHS versus NHS pretreated with anti-C1q antibody, anti-IgG antibody or anti-OmpU antibody (p < 0.05). Double asterisks indicate ΔompU mutant in NHS versus the ΔompU mutant harboring either vector control or the wild-type ompU clone or ompU/D114A clone in NHS (p < 0.05).