Table 1.
The strains and plasmids used in this study
| Strain/plasmid | Description/relevant characteristics | Reference No./source |
|---|---|---|
| E. coli strains | ||
| SM10λpir | thi thr leu tonA lacY supE recA:: RP4-2 TC:: Mu Km λpir | 35 |
| MC4100 | araD139Δ(lac)U169 strA thi | 36 |
| ΔompC | ΔompC derivative of MC4100 | 37 |
| V. cholerae strains | ||
| A1552 | O1 El Tor, Inaba, RifR | 30 |
| C6706 | O1 El Tor, Inaba, StrR | 38 |
| 569B | O1 Classical, Inaba, RifR | 39 |
| O395 | O1 Classical, Inaba, StrR | 40 |
| V:1/05 | Non-O1 Non-O139, clinically isolated (2005) | Swedish Institute of Infectious Diseases, Sweden |
| V:5/04 | Non-O1 Non-O139, clinically isolated (2004) | Swedish Institute of Infectious Diseases, Sweden |
| V:10/04 | Non-O1 Non-O139, clinically isolated (2004) | Swedish Institute of Infectious Diseases, Sweden |
| V:7/04 | Non-O1 Non-O139, environmentally isolated (2004) | Swedish Institute of Infectious Diseases, Sweden |
| ΔompU | ΔompU derivative of A1552 | This study |
| ΔompT | ΔompT derivative of A1552 | 41 |
| ΔompA | ΔompA derivative of A1552 | 30 |
| ΔtoxR | ΔtoxR derivative of A1552 | 41 |
| Plasmids | ||
| pBAD18 | Arabinose-inducible vector, CbR | 42 |
| pMMB66HE | IPTG inducible vector, CbR | 43 |
| pJET1.2 | Cloning vector, CbR | Fermentas |
| pBR322 | Cloning vector, CbR, TcR | 44 |
| pJET-ompU | pJET1.2, ompU gene from A1552 | This study |
| pMM-ompU | pMMB66HE, ompU gene from A1552 | This study |
| pMM-ompUD114A | pMM-ompU, D114A substitution | This study |