Fig. 4.

Histopathology of the lungs of WT, gzmA^−/−, gzmB^−/− and gzmAxB^−/− mice during K. pneumoniae pneumonia. Mice were infected with 4 × 10³ CFU K. pneumoniae intranasally and sacrificed at 6, 16 and 48 h after infection. a Semi-quantitative histology total score of lung slides is represented. Staining on representative lung slides of WT (b), gzmA^−/− (c), gzmB^−/− (d) and gzmAxB^−/− (e) mice 16 h after K. pneumoniae infection. HE. ×10. Scale bars: 200 μm. Box-and-whisker diagrams depict the smallest observation, lower quartile, median, upper quartile and largest observation (n = 7-8 per group at each time point). * p < 0.05, ** p < 0.01, *** p < 0.001 (Mann-Whitney U test).