Fig. 2.

Role of protein A in IL-1RII shedding by myeloid cells. THP-1 cells (a-c), monocytes (d, e) and neutrophils (f, g) were stimulated for 1 (a) or 2 h (b-g) with full-length protein A (SpA), GST (as a control), L. lactis SpA, L. lactis containing a control vector (L. lactis CV), wild-type S. aureus, the SpA^- mutant or media alone, and sIL-1RII in the supernatant was quantified by ELISA. a-c Data are presented as the mean and standard deviation of triplicate wells from 1 representative experiment out of 3. * p < 0.05; ** p < 0.01; *** p < 0.001, Student's t test. d-g Boxes and whiskers depict maximum and minimum values obtained from individual donors (4-5 per group) and the horizontal line represents the median for each group. * p < 0.05, one-way ANOVA-Friedman's test with Dunn's post hoc test.