Fig. 3.

Signaling cascades involved in the cleavage of IL-1RII. a- g THP-1 cells were stimulated with full-length protein A (SpA), the amino-terminal portion of protein A (indicated as EC), the polymorphic region of SpA (indicated as X), the IgG-binding domain D, its corresponding L17A mutant (indicated as L17A), GST (as a control), L. lactis SpA, L. lactis containing a control vector (L. lactis CV) or media alone in the absence (control) or presence of an ADAM17 inhibitor TAPI-I (a, b), an EGFR phosphorylation inhibitor AG1478 (e, f) and a erk1/2 phosphorylation inhibitor UO126 (g). The induction of sIL-1RII was determined at 1 (a, c-e, g) or 2 h (b, f) after stimulation by ELISA. Data are presented as the mean and standard deviation of triplicate wells from 1 representative experiment out of 3. * p < 0.05; ** p < 0.01; *** p < 0.001, Student's t test.