Peritoneal S. aureus infection. Mice were intraperitoneally inoculated with wild-type S. aureus, the SpA- mutant or PBS (control). IL-6 (a), IFN-γ (b), CXCL1 (c) and CXCL10 (d) levels in peritoneal fluid were quantified by ELISA. Each dot represents an individual mouse and horizontal lines show the median value in each group. * p < 0.05; ** p < 0.01, nonparametric Mann-Whitney test. The percentage of recruited neutrophils (e) and monocytes (f) as well as the ratio of neutrophils/monocytes (g) in the peritoneum at 4 h after inoculation was determined by flow cytometry. Boxes and whiskers depict maximum and minimum values obtained from individual mice (5 per group) and the horizontal line represents the median for each group. * p < 0.05, nonparametric Mann-Whitney test. h Bacterial counts in peritoneum at 2 and 4 h postinoculation. Each box represents an individual group of mice and horizontal lines show the median value for each group. * p < 0.05, nonparametric Mann-Whitney test.