Fig. 2.

Loss of Unc93b1 function increases cardiac damage and inflammation during acute CVB3 infection. a Myocarditis score, a composite of cardiac inflammation and necrosis, for Unc93b1^+/+ hearts (black circles) and Unc93b1^Letr/Letr hearts (white circles) at day 8 after infection. A score of ‘0’ indicates the absence of myocarditis while ‘4’ denotes pervasive necrosis and inflammation throughout the heart section (n ≥11 in each group). *** p < 0.001, 2-tailed unpaired Student's t test. b, c Representative histological heart sections. HE. ×4 (top panel), ×10 (bottom panel). Inflammation is visualized as condensed areas of nucleated cells (stained blue) while necrosis causes the appearance of areas of clearance within normal heart tissue (stained red) [32].