Fig. 5.

ARF3 regulates CpG ODN-induced NF-κB activation upstream of MyD88. a WT MyD88-overexpressing 293T cells were transiently transfected with various concentrations of ARF3T31N or ARF6T27N plus p5× NF-κB luciferase. After 24 h of transfection, NF-κB luciferase activities were measured. b The mTLR9-293T cells were transiently transfected with pcDNA3.1 or ARF3Q71L plasmids plus p5 × NF-κB luciferase with/without the MyD88-DN plasmid overnight, and then stimulated with 1.0 μM GpC ODN (negative control) or CpG ODN for 8 h. The NF-κB luciferase activities were then measured. Data represent the mean ± SD from 3 experiments.