Fig. 7.

Stable expression of ARF3T31N interferes with both the trafficking and proteolytic processing of TLR9. a RAW-pcDNA, RAW-ARF1T31N, RAW-ARF3 or RAW-ARF3T31N cells were treated with or without CpG ODN (1.0 μM) for 2 h. The cells were then fixed, permeabilized and labeled with anti-TLR9 (FITC: green) and anti-LAMP1 (DyLight 549: red) antibodies. These images are representative of 3 independent experiments. Scale bar: 5 μm. b The cell lysates of 293T cells, TLR9- cherry-expressing 293T cells transiently transfected with pcDNA3.1, ARF3-HA, ARF3T31N-HA or ARF3Q71L-HA plasmids incubated in the culture medium or a medium supplemented with 1.0 μM CpG ODN for 2 h were subjected to Western blot analysis with TLR9- (anti-cherry), ARF3- (anti-HA) or β-actin-specific antibodies. The bottom panel shows quantitative analysis by densitometry. Error bars denote mean ± SD of 3 experiments.