Fig. 8.

BWZ.NKR-P1B reporter cell analysis of Clr-b ligand function on type-I interferon (IFN)-treated stimulator cells. NIH3T3 cells (a), primary wild-type mouse embryonic fibroblast cells (b), primary Irf9^−/− mouse embryonic fibroblast cells (c), or primary Irf3^−/−Irf9^−/− mouse embryonic fibroblast cells (d) were used as stimulator cells for Ifnar1^−/− CD3ζ/NKR-P1B receptor-bearing BWZ.36 reporter cells, either untreated or treated overnight with 10³ U/mL IFN-α₄, in the absence or presence of blocking Clr-b mAb. Control Ifnar1^−/− BWZ.36 cells are not shown. Experiments were analyzed using ANOVA with Bonferroni's post hoc analysis comparing treated to untreated. The results are representative of at least 4 independent experiments, and the graphs show means ± SEM. OD, optical density. * p < 0.05, *** p < 0.001, **** p < 0.0001.