Fig. 1.

a Schematic illustration of the IAP2 expression constructs used in this study. Numbers indicate BIR motifs in the respective constructs and a dark grey box indicates the RING domain. MW of the respective expression constructs including the 3HA tag are indicated below each protein. b, c Co-immunoprecipitation studies of lysates from S2 cells that were transfected with the expression constructs indicated at the top of the gel, and immunoprecipitated with a monoclonal anti-HA antibody. Input and immunoprecipitate fractions were visualized with anti-HA and anti-myc antibodies as indicated. MW is shown for each blot in kilodaltons. MycDreddCA indicates a proteolytically inactive variant of MycDredd. IgH indicates the Ig heavy chain from immunoprecipitations (b).