Fig. 1.

Pyk2 is recruited to cell-associated anti-CR3 beads. a Serum-starved RAW 264.7 cells were activated with DMEM/10% hiFBS for 10 min and infected with CFSE-labeled beads coupled to albumin, rat IgG2b (control), α-CD11b Ab (anti-CR3) or IgG (MOI 15) for 30 min. After infection, the cells were fixed and immunostained with α-Pyk2 (1:50) and α-rabbit-Cy3 Ab. In addition, actin was stained using Phalloidin-Cy5. The regarded beads are indicated with arrows. Scale bars: 10 μm. b Normalized fluorescence intensity profiles of sites of bead attachment over a 5-µm distance (dashed lines in a). Intensities were normalized to maximal CFSE fluorescence and mean values of 10 intensity profiles are shown with error bars representing SEM.