Fig. 7.

TNF-α and IL-6 production in J774A.1 and RAW264.7 cells after HMGB1-mediated transfer of LTA. WT = Wild-type. J774A.1 (a, c) and RAW264.7 cells (b) were treated with LTA in the presence or absence of wild-type HMGB1 or HMGB1 A and B box proteins in OPTI-MEM for 20 h, after which the levels of TNF-α and IL-6 in the supernatants were measured. The experiment was repeated more than 3 times, and the mean ± SEM are shown. * p < 0.005 vs. LTA only: one-way ANOVA test with Bonferroni correction.