Generation and characterization of PGRP-SC1- and PGRP-SC2-specific mutants. a Schematic representation of PGRP-SC1a/b and PGRP-SC2 mutants. The gene map was adapted from FlyBase. The deleted segment replaced by the mini-white gene (black box) is indicated. For the PGRP-SC1a/b locus, the deletion starts at position 2R: 8,709,733 and ends at position 2R: 8,715,094 and also includes the CG14743 gene; for the PGRP-SC2 locus, the deletion starts at position 2R: 8,716,950 and ends at position 2R: 8,717,695. b-d Relative gene expression of PGRP-SC1 (b), PGRP-SC2 (c) and PGRP-LB (d) in different gut domains of axenic larvae of the three specific mutants compared to controls (WT). Means ± SD of three independent experiments. p > 0.05 (n.s.), ** p < 0.05 and *** p < 0.01 (Student's t test).