Fig. 5.

Impact of NET on cytokine secretion by macrophages (MØ) and dendritic cells (DC). CXCL8/IL-8 (a) and IL-6 (b) secretion by MØ cultured for 18 h at 37°C, with medium (Basal) or SN MSU-NET in the presence or absence of EI (10 µM) for 4 h. CXCL8/IL-8 (c) and IL-6 (d) production by DC cultured without NET (Basal) or with SN NET-MSU, SN unPMN, or LPS as a positive control (150 ng/mL). Data are depicted as the mean ± SEM of 3 independent experiments performed in triplicate. * p < 0.05 (two-way ANOVA with repeated measures, followed by Bonferroni's multiple-comparisons test).