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. 2019 Aug 21;18(19):2566–2579. doi: 10.1080/15384101.2019.1652046

Table 1.

The primers for RT-qPCR.

Gene Sequence
LINC00899 F: 5ʹ-ACTTCCAGAACCTGACGTGG-3’
R: 5ʹ-TGCTTTCAACCTCCGTTCCA-3’
RBL2 F: 5ʹ-TTCCCTGTCCAAGTCAATGT-3’
R: 5ʹ-CTGGGGAAATCTGTTGAATG-3’
FoxO F: 5ʹ-GCAGACCATCCAAGAGAACAA-3’
R: 5ʹ-TGTGGCTAAGTGAGTCCGAAG-3’
p21 F: 5ʹ-TGCAACTACTACAGAAACTGCTG-3’
R: 5ʹ-CAAAGTGGTCGGTAGCCACA-3’
p27 F: 5ʹ-CGATAGCTGTGTGCAAAGTAACT-3’
R: 5ʹ-CCATCTGCTGAGTGCTTTCTG-3’
Bcl-2 F: 5ʹ-GTCTTCGCTGCGGAGATCAT-3’
R: 5ʹ-CATTCCGATATACGCTGGGAC-3’
Bax F: 5ʹ-CATATAACCCCGTCAACGCAG-3’
R: 5ʹ-GCAGCCGCCACAAACATAC-3’
Vimentin F: 5ʹ-GCCCTAGACGAACTGGGTC-3’
R: 5ʹ-GGCTGCAACTGCCTAATGAG-3’
Annexin F: 5ʹ-CCACGGAGTACGTGTTCTCG-3’
R: 5ʹ-CCGCCCAGCAATATGAATCC-3’
GAPDH F: 5ʹ-ATGGAGAAGGCTGGGGCTC-3’
F: 5ʹ- AAGTTGTCATGGATGACCTTG −3’
U6 F: 5ʹ-GCTTCGGCAGCACATATACTAAAAT-3’
R: 5ʹ-CGCTTCACGAATTTGCGTGTCAT-3’

Notes: F, Forward; R, Reverse; RT-qPCR, reverse transcription quantitative polymerase chain reaction; LINC00899, long non-coding RNA LINC00899; RBL2, retinoblastoma-like protein 2; FoxO, forkhead box O; p21, protein 21; p27, protein 27; Bcl-2, B-Cell CLL/Lymphoma 2; Bax, BCL-2-associated X protein; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; U6, Ultima 6.