Figure 3.

LncRNA CRNDE as a ceRNA to regulate miR-33a. (a): Online analysis website predicts CRNDE subcellular localization; (b): FISH experiment verifies CRNDE subcellular localization; (c): RNA22 website predicts CRNDE binding sites to miR-33a; (d): Luciferase activity assay verifies the binding of CRNDE to miR-33a; (e): RIP assay detects the binding of CRNDE to Ago2; (f): RNA-pull-down assay detects the enrichment of miR-33a to CRNDE; * P < 0.05 vs the sh-NC group; the data are all measurement data, expressed as mean ± standard deviation, and the independent sample t-test was performed for comparisons between two groups for statistical analysis. The experiment was performed three times.