FIGURE 2. MOLECULAR ARCHITECTURE OF THE CRISPR-CAS9 COMPLEX,

from cryoelectron microscopy experiments and computational modeling.^4,10 The Cas9 protein (gray) is bound to a guide RNA segment (magenta) and a matching DNA sequence (the complementary strand is dark blue; the noncomplementary strand, light blue; and the PAM segment, yellow). The Cas9 protein contains two nuclease domains, HNH (green) and RuvC (pink), which cleave the complementary and noncomplementary DNA strands, respectively.