The highest expression of FBP1 is identified in PCa PC-3 and DU-145 cell lines. A, mRNA expression of FBP1 in human normal prostatic epithelial cell line (RWPE-1) and PCa cell lines (DU-145, PC-3, RM-1, LNCaP and IA8) detected by RT-qPCR. B, western blot analysis of FBP1 protein level in human normal prostatic epithelial cell line (RWPE-1) and PCa cell lines (DU-145, PC-3, RM-1, LNCaP and IA8). The band intensity was assessed. The above data were measurement data, which were presented as mean ± standard deviation and analyzed by one-way ANOVA. The multiple comparison was analyzed by post-hoc Tukey test. The experiment was repeated in triplicates. * p < 0.05 vs. the RWPE-1 cell line; # p < 0.05 vs. the DU-145 cell line. RT-qPCR, reverse transcription quantitative polymerase chain reaction; PCa, prostate cancer; FBP1, fructose-bisphosphatase 1; ANOVA, analysis of variance.