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. 2019 Aug 25;18(19):2432–2446. doi: 10.1080/15384101.2019.1648956

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Figure 5. — FBP1 gene silencing up-regulates mRNA expression of ERK, N-cadherin and Vimentin and down-regulates that of E-cadherin. A, mRNA expression of FBP1, E-cadherin, Vimentin, N-cadherin and ERK in DU-145 cells detected by RT-qPCR. B, mRNA expression of FBP1, E-cadherin, Vimentin, N-cadherin and ERK in PC-3 cells measured by RT-qPCR. The mRNA expression determined by RT-qPCR was measurement data, which were presented as mean ± standard deviation and analyzed by one-way ANOVA. The multiple comparison was analyzed by post-hoc Tukey test. The experiment was repeated three times. *, p < 0.05 vs. the blank group; # p < 0.05 vs. the U0126 group; & p < 0.05 vs. the PD98059 group. NC, negative control; FBP1, fructose-bisphosphatase 1; ERK, extracellular regulated protein kinases; PCa, prostate cancer; RT-qPCR, reverse transcription quantitative polymerase chain reaction; ANOVA, analysis of variance.