FIG 1.

Genomic context of 45 experimentally characterized Acr representative genes. In total, 38 genomic loci are shown. Each locus contains at least one Acr gene (red arrows). There are 26 loci that also contain Aca genes (cyan arrows) with Pfam HTH or DUF1870 domains. Seven AcrIIA genes do not have neighboring Aca genes. The five AcrVA genes form four loci that also contain AcrIF genes, an AcrIC gene, and functional unknown genes (gray arrows) but no Aca genes. The plots were made with the Gene Graphics server (38). *, AcrF11 homologs were found to be next to other HTH domain-containing proteins as well, which were named Aca4 through Aca7 in reference 11. The archaeal viral AcrID1 has an upstream HTH protein, which has never been officially named and is named Aca8 here.