TABLE 2.
Sixteen experimentally characterized Acr-Aca loci in bacterial genomes
| Bacterial species | RefSeq genome ID | Published Acr-Aca loci | Presence of complete CRISPR-Cas and self-targeting spacer |
|---|---|---|---|
| Listeria monocytogenes J0161 | GCF_000168635 | AcrIIA2-AcrIIA1 | Yes |
| Listeria monocytogenes serotype 7 strain SLCC2482 | GCF_000210795 | AcrIIA2-AcrIIA3-AcrIIA1 | Yes |
| Listeria monocytogenes J0161 | GCF_000168635 | AcrIIA4-AcrIIA1 | Yes |
| Pseudomonas otitidis | GCF_900111835 | AcrIE5-Aca1 | Yes |
| Pseudomonas aeruginosa WH-SGI-V-07059 | GCF_001450485a | AcrIF4-AcrIE3-Aca1 | No self-target spacer |
| Haemophilus parainfluenzae | GCF_001053575a | AcrIIC4-Aca2 | No self-target spacer |
| Pseudomonas citronellolis | GCF_001654435a | AcrIE4-IF7-Aca1 | No self-target spacer |
| Pseudomonas aeruginosa strain C 1426 | GCF_000412555a | AcrIF11-Aca1 | No self-target spacer |
| Pseudomonas aeruginosa strain Jp54 | GCF_003836565b | AcrIF6-Aca1 | No self-target spacer |
| Vibrio parahaemolyticus | GCF_000736335a | AcrIF9-Aca1 | No complete CRISPR-Cas |
| Shewanella xiamenensis | GCF_000712635a | AcrIF10-Aca1 | No complete CRISPR-Cas |
| Neisseria meningitidis | GCF_001066195a | AcrIIC1-Aca3 | No complete CRISPR-Cas |
| Neisseria meningitidis 992008 | GCF_000724735a | AcrIIC2-AcaIIC3-Aca3 | No complete CRISPR-Cas |
| Simonsiella muelleri ATCC 29453 | GCF_000163775a | AcrIIC5-Aca2 | No complete CRISPR-Cas |
| Pseudomonas aeruginosa strain S708_C14_RS | GCF_002136415b | AcrIE6-Aca1 | No complete CRISPR-Cas |
| Pseudomonas aeruginosa strain 359 | GCF_002312455b | AcrIF12-Aca4 | No complete CRISPR-Cas |
a
These genomes are contained in the version of the RefSeq database that we have mined but have no self-targeting spacers according to the work of Watters et al. (12).
b
These genomes were not in the version of the RefSeq database that we have mined, so we downloaded them separately from NCBI. All these genomes were analyzed using CRISPRCasFinder to confirm the presence of complete CRISPR-Cas systems and using our in-house programs to confirm the presence of self-targeting spacers.