Fig. 1. An overview of the current study.

(A and B) Obtaining gene expression data generated from DLPFC (CMC data) and later CNON data as an independent validation. (C) Transcriptional network reconstruction based on the RNA-seq data using algorithm for reconstruction of accurate cellular networks (ARACNe) and identification of TCF4 as a disease-relevant MR. (D) Knockdown of TCF4 in hiPSCs derived from a patient with SCZ and two unaffected individuals. (E) Differentiation of hiPSC into neural progenitor cells (NPCs) and glutamatergic neurons (Glut_Ns). (F) RNA-seq of the generated neuronal cells with and without TCF4 knockdown. (G) Downstream inferences and analysis of the conducted experiments.