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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: J Orthop Res. 2019 Jun 24;37(10):2089–2103. doi: 10.1002/jor.24384

Figure 5. Notch inhibition impairs resorptive activity in vivo without impacting TRAP+ cell numbers and gene expression.

Figure 5.

profiles. (A) CTX-1 levels in serum obtained from 3-month-old male and female WT and dnMAML mice were significantly reduced in dnMAML mice suggesting reduced resorptive activity. No differences in serum osteocalcin (OCN) levels were observed between the 2 groups suggesting comparable osteoblast activity. TRAP+ cell numbers and expression profile of osteoclastic marker genes (TRAP, CTSK, MMP-9 and Wnt10b) were analyzed in (B) 3-month-old males and (C) 3-month-old females using TRAP staining and qRT-PCR, respectively. There were no significant differences in TRAP+ cell numbers or gene expression profiles between these groups. Values represented are Mean ± S.D and P value was calculated using Student t-test (*P<0.05).