Fig. 1. End1/E6E7 cells express IL-22 receptor complex.
(A) Total cellular RNA extracted from HepG2, L02 and End1/E6E7 cells was subjected to the RT-PCR with the primers specific for IL-22R (IL-22R1 and IL-10R2). Amplified PCR products were displayed on 2% agarose gel. Negative control: no cellular RNA but water was used as a template. (B, C) End1/E6E7 cells were treated with IL-22 at the indicated concentrations. Cells viability was assessed by MTT assay (B) and Annexin V/7-AAD assay (C) 72 h post IL-22 treatment.